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People
Krishna Saharan
Position
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Post Doctoral fellow |
Current site of work |
Fondazione Edmund Mach |
Street, number |
via Mach, 1 |
City |
S. Michele all'Adige TN |
Postal code |
38010 |
Country |
Italy |
E-mail |
krishana.saharan@iasma.it, microkrishna82@yahoo.co.in |
Phone |
+39 0461 615 502 |
Mobile phone |
+39 331 8056 702 |
Degree, Msc, or PhD; when; where; on what subject; title of the thesis |
PhD, 2011; in Biotechnology, thesis titled “Studies on Production, Formulation and Agronomical application of Fluorescent Pseudomonad strain-R62” under the supervisions of Dr A. Prakash at Department of Biotechnology and Bioinformatics centre (BU, Bhopal, 20% research work) and Dr. Vikram Sahai at Department of Biochemical Engineering and Biotechnology (IIT Delhi, 80% research work), India. |
Expertise |
Culture medium development for bacteria and fungus and various fermentation techniques: Batch and Fed-batch culture Bio-formulation, Bio-control and Bio-fertilizer agents |
Professional societies |
Member of Association of Microbiologists of India (AMI) |
Research experience, training, professional stages |
May 2011 - Present: Post Doc “To study the gene expression of a microorganism in presence of an activator in the formulation and some carriers to prolong the survival under stressing conditions”. Istituto Agrario di San Michele all’Adige, Trento, Italy. (P.I. Dr. Ilaria Pertot).
July 2009 – Nov. 2009: Short stay for training abroad participating in the project “Plant innate immunity through their plant microbial interaction elicitors” under the supervisions of Prof. Thomas Boller and Prof Andres Wiemken at Botanical Institute of Basel, Switzerland.
July 2006 - Oct 2010: Ph.D research activities were to develop a modified and optimized synthetic Schlegel’s medium for higher cell mass and metabolites (siderophore, IAA and 2,4 DAPG) production by fluorescent pseudomonad R62 in shake flasks and in 5 L batch bioreactor, with the objective of developing inorganic carriers based bio-inoculant formulations and to test their agronomical applications on various crops and check their antagonistic effect on various phytopathogens. Innovative research oriented toward to the develop technologies for reducing chemical inputs in agriculture and by using low impact bio fertilizers and bio-control to suppress the pathogens on plants.
Jan. 2005 - April 2011: Associated with the Indo Swiss Collaboration of Biotechnology (ISCB) sponsored project titled “Mass Scale multiplication of PGPRs” at Indian Institute of Technology Delhi (IITD) India, under the supervision of Prof V S Bisaria & Prof V Sahai.
Jan. 2005 - June 2006: Traineeship at the Indian Institute of Technology, Delhi, work on cultivation and formulation of plant growth promoting rhizobacteria such as pseudomonas spp.(bio-fertilizer), Trichoderma spp.(bio-control) and Melanocarpus albomyces, xylanase enzyme producing fungus in shake flask & bioreactor. (PI: Dr. Vikram Sahai and Co PI: Prof V. S. Bisaria). |
Website |
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Current research area |
Biofertilizer and microbial biocontrol agents, genetic basis of mycoparasitism, gene expression and regulation, formulation, shelf life under stressing conditions |
Publication List (last five years):
Krishna Saharan, M.V.R.K. Sarma, Rashmi Srivastava, A.K. Sharma, B.N. Johri, A. Prakash, Vikram Sahai, V.S. Bisaria (2010). Development of non-sterile inorganic carrier-based formulations of fluorescent pseudomonad R62 and R81 and evaluation of their efficacy on agricultural crops. Applied Soil Ecology. 46: 251-258.
Krishna Saharan, M.V.R.K. Sarma, A. Prakash, B.N. Johri, Vikram Sahai and V.S. Bisaria. (2010). Shelf-life enhancement of bio-inoculant formulation by optimizing the trace ions in the culture medium for production of DAPG using fluorescent pseudomonad R62. Enzyme and Microbial Technology.48:33-38.
Krishna Saharan, M.V.R.K. Sarma, N. Mathimaran, V.S. Bisaria, ,V. Sahai, A. Dominguez, A. Wiemken, T. Boller, (2010). Plant growth-promoting pseudomonad produce elicitors which induce ethylene production in host plants. Special Abstracts / Journal of Biotechnology, 150S (2010) S1–S576. doi:10.1016/j.jbiotec.2010.09.883.
Krishna. Saharan, M.V.R.K. Sarma, A.S. Roesti,A. Prakash, B.N. Johri, M. Aragno,V.S. Bisaria and V. Sahai (2010). Cell Growth and Metabolites Produced by Fluorescent Pseudomonad R62 in Modified Chemically Defined Medium. World Academy Science, Engineering and Technology 67: 867-871.
M.V.R.K Sarma, Krishna Saharan*, Lalit Kumar, Ashwani Gautam, Avhijeet Kapoor, Nishant Srivastava, Vikram Sahai and V.S Bisaria (2010) Process Optimization for Enhanced Production of Cell Biomass and Metabolites of Fluorescent Pseudomonad R81. World Academy Science, Engineering and Technology 65: 997-1001.
Sarma M.V.R.K, Krishna Saharan*, Prakash A, Bisaria VS, Sahai V., (2009). Application of fluorescent pseudomonad inoculants formulations on Vigna-mungo through field trial. International Journal of Biological science and Life science 36: 25-31.
Gupta V, Krishna Saharan*, Lalit K, Gupta R, Sahai V, Mittal A. (2008). Spectrophotometric Ferric Ion Biosensor from Pseudomonas fluoresces Culture. Biotechnology and Bioengineering, 100 (2): 284-296.
M.V.R.K. Sarma, Vinod Kumar, Krishna Saharan*, Rashmi Srivastava, A.K. Sharma, A. Prakash, Vikram Sahai, V.S. Bisaria (2011).Application of inorganic carrier-based formulations of fluorescent pseudomonads and Piriformospora indica on tomato plants and evaluation of their efficacy. Journal of Applied Microbiology, accepted in May, 2011.
Vinod Kumar, M.V.R.K Sarma, Krishna Saharan*, Rashmi Srivastava, A.K. Sharma, Vikram Sahai, V.S. Bisaria (2011).Effect of formulated root endophytic fungus Piriformospora indica and plant growth promoting rhizobacteria fluorescent pseudomonads R62 and R81 on Vigna mungo. Submitted.
*Equal contribution along with first author.
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